Stability and Endopeptidase Activity of Botulinum Neurotoxin Type A Light Chain
نویسندگان
چکیده
منابع مشابه
Endopeptidase activities of botulinum neurotoxin type B complex, holotoxin, and light chain.
Botulinum neurotoxin (BoNT) serotype B (BoNT/B) is one of the serotypes of BoNT that causes deadly human botulism, though it is used clinically for treatment of many neuromuscular diseases. BoNT/B is produced by Clostridium botulinum, and it is secreted along with a group of neurotoxin-associated proteins (NAPs) in the form of a BoNT/B complex. The complex dissociates into a 150-kDa holotoxin a...
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Botulinum neurotoxin A (BoNT/A) has intrinsic endoprotease activity specific for SNAP-25, a key protein for presynaptic neurotransmitter release. The inactivation of SNAP-25 by BoNT/A underlies botulism, a rare but potentially fatal disease. There is a crucial need for a rapid and sensitive in vitro serological test for BoNT/A to replace the current in vivo mouse bioassay. Cleavage of SNAP-25 b...
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Highly purified recombinant zinc-endopeptidase light chain of the botulinum neurotoxin serotype A underwent autocatalytic proteolytic processing and fragmentation. In the absence of added zinc, initially 10-28 residues were cleaved from the C-terminal end of the 448-residue protein followed by the appearance of an SDS-stable dimer and finally fragmentation near the middle of the molecule. In th...
متن کاملCharacterization of antibody titer and immunogenic feature of light chain of botulinum neurotoxin type A
The botulinum neurotoxins (BoNTs), the most toxic biological compounds, are known to cause muscle paralysis. These toxins are enzymatic properties which inhibit the release of the acetylcholine. The purposes of this study are the recombinant expression of light chain (LC) of botulinum toxin types A (rBoNT/A-LC) with high purity, the evaluation of its antibody titer yield and its immunogenicity,...
متن کاملNeurologic uses of botulinum neurotoxin type A
This article reviews the current and most neurologic uses of botulinum neurotoxin type A (BoNT-A), beginning with relevant historical data, neurochemical mechanism at the neuromuscular junction. Current commercial preparations of BoNT-A are reviewed, as are immunologic issues relating to secondary failure of BoNT-A therapy. Clinical uses are summarized with an emphasis on controlled clinical tr...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2007
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.21.6.a1006-c